iQ-Check Vibrio PCR Detection Kit – Bio-Rad Laboratories

Bio-Rad Laboratories (Singapore) Pte. Ltd.

The iQ-Check Vibrio PCR Detection Kit is a multiplex test based on gene amplification and detection by real-time PCR.

This test allows the qualitative detection of V. cholerae, V. parahaemolyticus, and V. vulnificus in food samples previously enriched by culture. The main targeted matrices are seafood, including fish and shellfish (mollusks and crustaceans).

Highly Specific and Reliable

The iQ-Check Vibrio Kit uses an optimized system of primers and probes to ensure high specificity. It is designed as a multiplex reaction that includes an internal inhibition control that is amplified in parallel with the target DNA for a reliable result (negative result validation). The iQ-Check Free DNA Removal Solution completes and optimizes the method by removing free DNA in the sample.

Maximum Flexibility

Developed as an open and flexible system, the iQ-Check Vibrio Kit can be used for up to 94 samples. Reactions can be run on low- or high-throughput Bio-Rad instruments, depending on the laboratory’s needs. Multiple iQ-Check Assays can be run in parallel on the same plate.

A Complete Solution

Bio-Rad offers a complete solution with standardized reagents, such as enrichment media, PCR kits, confirmation agars, and PCR detection systems, in addition to software for automated analysis specific to each test.

 

The iQ-Check Salmonella II PCR Detection Kit is based on the fast, sensitive, and proven technology of real-time PCR. The kit is intended for detecting Salmonella spp. in animal feed and environmental samples, as well as in food samples such as poultry, eggs, and raw meats.
Due to the sensitivity and specificity of PCR, results can be obtained in little as 12 hr for raw meat samples and in 20 hr for all other samples following a single enrichment in a nonselective medium.

Highly Specific and Reliable
The iQ-Check Salmonella II Kit uses an optimized system of primers and probes to ensure high specificity and eliminate cross-reactions. It is designed as a multiplex reaction that includes an internal inhibition control that is amplified in parallel with the target DNA for a reliable result.

Maximum Flexibility
Developed as an open and flexible system, the iQ-Check Salmonella II Kit can be used for up to 94 samples. Reactions can be run on low- or high-throughput Bio-Rad instruments, depending on the laboratory’s needs. Different iQ-Check assays can be run in parallel on the same plate.

Validated Protocols
The iQ-Check Salmonella II protocol has been validated by several certification bodies. These validation studies have shown that the kit is equivalent to or better than the various reference methods evaluated and provides results in much less time.

A Complete Solution

Bio-Rad offers a complete solution with standardized reagents, such as enrichment media, PCR kits, confirmation agars, and PCR detection systems, in addition to software for automated analysis specific to each test.

Kit Components
•Lysis reagent for DNA extraction
•Fluorescent probes
•PCR amplification mix
•Positive and negative PCR controls

RAPID’L.mono Medium is a selective chromogenic medium for the direct detection and enumeration of Listeria monocytogenes and Listeria spp. in food and environmental samples.
Key Benefits
• Fast
o L. monocytogenes detection 24 hr after enrichment*
o Listeria spp. detection 24 and 48 hr after enrichment
o L. monocytogenes enumeration in 24 hr without confirmation**
o L. monocytogenes confirmation in 24 hr after using the iQ-Check® Listeria monocytogenes II Kit
• Simple and Cost Effective
o Only 1 broth, and 1 plate required for each sample
o Easy confirmation
• Easy-to-Read Chromogenic Reaction
o L. monocytogenes (PIPLC+/xylose-) forms blue colonies
o L. ivanovii (PIPLC+/xylose+) formsblue-green colonies with distinct yellow halos
o Other Listeria species form white colonies
• Multipurpose Chromogenic Medium
o RAPID’L.mono is the only chromogenic method to be validated for L. monocytogenes and Listeria spp. detection on the same plate
o Can also be used for the enumeration or confirmation of L. monocytogenes
• Validation
o Certified NF VALIDATION according to the ISO 16140 standard and validated by AOAC
* Within the scope of ISO 16140 NordVal validation, no confirmation is required.
** Within the scope of the AFNOR validation, confirmation is not required when the presence of L. monocytogenes has been confirmed at the detection stage.

 

 

Intended Use
RAPID’Salmonella Medium is a sensitive chromogenic agar used for the detection and enumeration of Salmonella spp. in products that are intended for human and animal consumption.
Key Benefits
• Fast results
o Less than 42 hr to detect Salmonella spp. when using the short protocol
o Fast confirmation (less than 1 minute)
• 1 broth and 1 plate
o Saves money
o Saves handling time
• Low false positive rate (<0.3% for all matrices)
o Saves on unnecessary confirmations
• Meets regulatory expectations
o Detects motile and non-motile Salmonella, as well as lactose-positive Salmonella, Salmonella Typhi, and Salmonella Paratiphy
o Certified NF VALIDATION according to the ISO 16140 standard and validated by AOAC

 

Introducing Bio-Rad’s newest culture media, RAPID’B.cereus
• Unique — allows enumeration of all pathogenic B. cereus species, including
Bacillus cytotoxicus
• Quick turnaround — produces results in under 24 ± 3 hours with no
confirmation required
• Easy interpretation — facilitates clear visualization of red colonies surrounded by
an opaque halo on a blue background
• Flexible — enables surface inoculation and pour plate protocols with storage up
to 72 hours at 2–8°C prior to reading
• Certified International AFNOR validation

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